4Z8Y
Crystal structure of Rab GTPase Sec4p mutant - S29V
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2008-12-13 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 31.720, 75.450, 66.230 |
Unit cell angles | 90.00, 91.58, 90.00 |
Refinement procedure
Resolution | 28.300 - 1.900 |
R-factor | 0.23874 |
Rwork | 0.236 |
R-free | 0.29840 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1g16 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.610 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 66.200 | 2.000 |
High resolution limit [Å] | 1.870 | 1.870 |
Rmerge | 0.052 | 0.285 |
Number of reflections | 23244 | |
<I/σ(I)> | 9.1 | 2.3 |
Completeness [%] | 94.8 | 98.3 |
Redundancy | 1.7 | 1.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | BATCH MODE | 293.15 | After initial crystal screening, small crystals were obtained in 14% PEG3350, 200mM Zinc Acetate. After refinement, large single crystals were obtained in 14% PEG4000, 50mM Zinc Acetate. |