4Z8F
Fab structure of antibody S1-15 in complex with ssDNA DNA, 5'-p5(dT)p-3'
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 113 |
Detector technology | CCD |
Collection date | 2014-12-20 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.9794 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 77.585, 77.585, 156.267 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.000 - 1.750 |
R-factor | 0.209 |
Rwork | 0.208 |
R-free | 0.24000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4odt |
RMSD bond length | 0.009 |
RMSD bond angle | 1.284 |
Data reduction software | HKL-2000 (v703c3) |
Data scaling software | SCALA |
Phasing software | PHASER (2.5.6) |
Refinement software | REFMAC (5.80073) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 1.810 |
High resolution limit [Å] | 1.750 | 1.750 |
Rmerge | 0.610 | |
Number of reflections | 46712 | |
<I/σ(I)> | 36 | 5.96 |
Completeness [%] | 99.9 | 100 |
Redundancy | 14.5 | 14.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 289.15 | 0.075M magnesium chloride, 0.1M HEPES pH 7.5, 28% (v/v) PEG 550 MME |