4Z66

Nucleosome disassembly by RSC and SWI/SNF is enhanced by H3 acetylation near the nucleosome dyad axis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2009-04-02
Detector	NOIR-1
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	105.730, 109.630, 181.110
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.870 - 2.500
Rwork	0.254
R-free	0.29580
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1p3l
RMSD bond length	0.006
RMSD bond angle	1.050
Data reduction software	d*TREK
Data scaling software	d*TREK (9.9.1LDz)
Refinement software	CNS

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	42.870	42.870	2.590
High resolution limit [Å]	2.500	5.380	2.500
Rmerge	0.096	0.056	0.412
Total number of observations	348947	24383	40781
Number of reflections	69124
<I/σ(I)>	6.9	17.9	2.6
Completeness [%]	94.0	83.5	99.8
Redundancy	4.95	3.47	5.59

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	292.15	potassium chloride, potassium cacodylate, manganese chloride