4Z5T
The nucleosome containing human H3.5
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-07-14 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 1.000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 104.891, 109.129, 174.492 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.812 - 2.800 |
R-factor | 0.2298 |
Rwork | 0.228 |
R-free | 0.26940 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3av2 |
RMSD bond length | 0.005 |
RMSD bond angle | 0.815 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.900 |
High resolution limit [Å] | 2.800 | 6.030 | 2.800 |
Rmerge | 0.094 | 0.054 | 0.500 |
Rmeas | 0.100 | 0.057 | 0.543 |
Rpim | 0.032 | 0.018 | 0.207 |
Total number of observations | 447587 | ||
Number of reflections | 50581 | ||
<I/σ(I)> | 10.2 | ||
Completeness [%] | 99.5 | 98.8 | 96.5 |
Redundancy | 8.8 | 10.2 | 6.4 |
CC(1/2) | 0.998 | 0.722 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | potassium cacodylate, potassium chloride, manganese chloride |