4Z46
X-ray structure of the bis-platinum lysozyme adduct formed in the reaction between the protein and the two drugs Cisplatin and Oxaliplatin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-06-26 |
| Detector | RIGAKU SATURN 944+ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 77.318, 77.318, 37.325 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 54.670 - 1.850 |
| R-factor | 0.1584 |
| Rwork | 0.155 |
| R-free | 0.21801 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.842 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.000 | 1.880 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.095 | 0.471 |
| Number of reflections | 10100 | |
| <I/σ(I)> | 13.8 | 3.1 |
| Completeness [%] | 99.4 | 92.2 |
| Redundancy | 5.8 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.6 M sodium nitrate, 0.1 M sodium acetate pH 4.4, 20 %ethylene glycol |
