4Z2M
Crystal structure of human SPT16 Mid-AID/H3-H4 tetramer FACT Histone complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-07-18 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 0.9000 |
| Spacegroup name | P 21 2 21 |
| Unit cell lengths | 65.442, 69.784, 187.853 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.963 - 2.981 |
| R-factor | 0.196 |
| Rwork | 0.190 |
| R-free | 0.24650 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2cv5 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.252 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.980 |
| Number of reflections | 17819 |
| <I/σ(I)> | 17.4 |
| Completeness [%] | 97.7 |
| Redundancy | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 10% PEG400, 0.9-1.1 M Imidazole, 100-150 mM L-histidine |
