4Z0X
Structure of Hepatitis C Virus Envelope glycoprotein E2 antigenic region 434-446 bound to the broadly neutralizing antibody HC26AM
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 298 |
Detector technology | CCD |
Collection date | 2014-09-10 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.075 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 50.720, 51.070, 69.660 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.190 - 2.000 |
R-factor | 0.1962 |
Rwork | 0.190 |
R-free | 0.24970 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB entries 4JZO 4hjo 3QOT 4DN3 (VH) & 4AIX 4ajo 4qhk 4DAG (VL) |
RMSD bond length | 0.008 |
RMSD bond angle | 1.004 |
Data reduction software | HKL-2000 |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 41.190 |
High resolution limit [Å] | 2.000 |
Rmerge | 0.145 |
Number of reflections | 15612 |
<I/σ(I)> | 14.8 |
Completeness [%] | 96.0 |
Redundancy | 12.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.2 | 298 | Sodium Phosphat monobasic, potassium phosphate dibasic, sodium phosphate dibasic/citric acid |