4YZY
Crystal structures reveal transient PERK luminal domain tetramerization in ER stress signaling
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-10-01 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9795 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 90.770, 90.770, 75.220 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 54.347 - 3.200 |
R-factor | 0.291 |
Rwork | 0.290 |
R-free | 0.30500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4yzs |
RMSD bond length | 0.004 |
RMSD bond angle | 1.065 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX (dev_1448) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 54.347 | 3.300 |
High resolution limit [Å] | 3.200 | 3.200 |
Number of reflections | 11682 | |
<I/σ(I)> | 12.2 | 2.6 |
Completeness [%] | 99.7 | 99.7 |
Redundancy | 5.2 | 6.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.1 M MES/imidazole (pH 6.5), 0.09 sodium phosphate salts (NPS) mix (containing 0.03 M of each NaN03, Na2HPO4, (NH4)2SO4), 12.5% w/v PEG1000, 12.5% w/v PEG3350, 20% v/v MPD. |