4YZS

Crystal structures reveal transient PERK luminal domain tetramerization in ER stress signaling

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-07-13
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.2148, 1.2152, 0.9795
Spacegroup name	P 41 21 2
Unit cell lengths	83.900, 83.900, 186.240
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	75.600 - 3.140
R-factor	0.2419
Rwork	0.240
R-free	0.28740
Structure solution method	MAD
RMSD bond length	0.004
RMSD bond angle	1.029
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	SHELXDE
Refinement software	PHENIX ((phenix.refine: dev_1448))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	75.600	3.200
High resolution limit [Å]	3.100	3.100
Number of reflections	22114
<I/σ(I)>	32.3	4.4
Completeness [%]	99.9	99.5
Redundancy	10.4	11

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.1 M Tris (pH 8.5), 0.2 M MgCl2, 25% w/v PEG3350, 7% glycerol