4YXP

The structure of the folded domain of the signature multifunctional protein ICP27 from herpes simplex virus-1 reveals an intertwined dimer.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-10-12
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.92
Spacegroup name	P 21 21 2
Unit cell lengths	99.330, 133.620, 44.970
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.553 - 1.920
R-factor	0.1699
Rwork	0.168
R-free	0.20260
Structure solution method	SAD
RMSD bond length	0.004
RMSD bond angle	0.798
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHENIX
Refinement software	PHENIX (dev_1977)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.553	1.989
High resolution limit [Å]	1.920	1.920
Rmerge	0.061	0.640
Number of reflections	46524
<I/σ(I)>	19.02	2.86
Completeness [%]	100.0	99
Redundancy	6.6	6.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	277	Crystals grown from reservoir solutions consisting of 0.09 M (NaF, NaBr, NaI), 0.1 M (Imidazole, MES) buffer system pH 6.5, 30% (Ethylene glycol, PEG 8K) [Morpheus HT96 B2 Molecular Dimensions] and 0.12 M (1,6-Hexanediol, 1-Butanol 1,2-Propanediol (racemic), 2-Propanol, 1,4-Butanediol, 1,3-Propanediol), 0.1 M (Imidazole, MES) buffer system pH 6.5, 30% (Ethylene glycol, PEG 8K) [Morpheus HT96 D2 Molecular Dimensions]