4YVS
crystal structure of the virus-like particle of a c4 strain EV71
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2012-10-02 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97853 |
| Spacegroup name | P 42 3 2 |
| Unit cell lengths | 349.746, 349.746, 349.746 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.964 - 3.650 |
| R-factor | 0.2721 |
| Rwork | 0.271 |
| R-free | 0.30030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1pov |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.001 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: dev_1839)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.780 |
| High resolution limit [Å] | 3.650 | 7.850 | 3.650 |
| Rmerge | 0.150 | 0.047 | |
| Rmeas | 0.163 | 0.054 | |
| Rpim | 0.101 | 0.025 | 0.741 |
| Total number of observations | 227650 | ||
| Number of reflections | 72397 | ||
| <I/σ(I)> | 5.7 | ||
| Completeness [%] | 89.2 | 84.7 | 84 |
| Redundancy | 3.1 | 3.9 | 2.5 |
| CC(1/2) | 0.997 | 0.586 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 290 | 0.1M Imidazole, 1M sodium acetate |
