4YPH
Crystal Structure of MutY bound to its anti-substrate with the disulfide cross-linker reduced
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-03-13 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 37.636, 83.757, 142.735 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.370 - 2.320 |
| R-factor | 0.1905 |
| Rwork | 0.187 |
| R-free | 0.25890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1rrq |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.367 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 83.760 | 2.450 |
| High resolution limit [Å] | 2.320 | 2.320 |
| Rmerge | 0.855 | |
| Number of reflections | 20065 | |
| <I/σ(I)> | 8.8 | 1.5 |
| Completeness [%] | 98.7 | 100 |
| Redundancy | 3.5 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 100 mM Tris, pH 8.0, 250 mM NaOAc, 29% (wt/vol) PEG 4000, 25 mM TCEP |
