4YP2
Cleavage of nicotinamide adenine dinucleotides by the ribosome inactivating protein from Momordica charantia
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-02-12 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.979 |
| Spacegroup name | H 3 |
| Unit cell lengths | 130.100, 130.100, 37.570 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 37.557 - 1.350 |
| R-factor | 0.1417 |
| Rwork | 0.140 |
| R-free | 0.16820 |
| Structure solution method | MIR |
| Starting model (for MR) | 1mom |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.156 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.560 | 1.390 |
| High resolution limit [Å] | 1.350 | 1.350 |
| Rmerge | 0.027 | 0.810 |
| Number of reflections | 51799 | |
| <I/σ(I)> | 16.8 | 1.4 |
| Completeness [%] | 99.4 | 99.6 |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | PEG 4000, sodium phosphate |
