4YM7
RNA polymerase I structure with an alternative dimer hinge
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-11-15 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.07392 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 619.480, 306.620, 251.780 |
| Unit cell angles | 90.00, 97.50, 90.00 |
Refinement procedure
| Resolution | 49.685 - 5.500 |
| R-factor | 0.1963 |
| Rwork | 0.196 |
| R-free | 0.23550 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4c2m |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.391 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 5.640 |
| High resolution limit [Å] | 5.500 | 5.500 |
| Rmerge | 0.220 | 0.600 |
| Number of reflections | 149610 | |
| <I/σ(I)> | 6.6 | 2.5 |
| Completeness [%] | 98.9 | 91 |
| Redundancy | 6.1 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 10% PEG 6000, 3% MPD, 100 mM Hepes, 300 mM di-ammonium-tartrate, 3 mM TCEP |
