4YJT
THE KINASE DOMAIN OF HUMAN SPLEEN TYROSINE (SYK) IN COMPLEX WITH GTC000233
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-04-28 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.933 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.328, 87.214, 40.230 |
| Unit cell angles | 90.00, 92.39, 90.00 |
Refinement procedure
| Resolution | 27.530 - 1.520 |
| R-factor | 0.176 |
| Rwork | 0.174 |
| R-free | 0.20830 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | In-house SYK model |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.020 |
| Data reduction software | MOSFLM (6.2.4) |
| Data scaling software | SCALA (3.2.5) |
| Refinement software | BUSTER (2.11.2) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 27.535 | 29.553 | 1.520 |
| High resolution limit [Å] | 1.440 | 4.550 | 1.440 |
| Rmerge | 0.055 | 0.036 | 0.501 |
| Total number of observations | 180152 | 5209 | 26188 |
| Number of reflections | 48894 | ||
| <I/σ(I)> | 13.4 | 32.1 | 2.3 |
| Completeness [%] | 99.9 | 96.2 | 100 |
| Redundancy | 3.7 | 3.4 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 30% Jeffamine ED-2001, 10% Glycerol, 0.1M MES pH6.0, 5mM TCEP |
