4YJT

THE KINASE DOMAIN OF HUMAN SPLEEN TYROSINE (SYK) IN COMPLEX WITH GTC000233

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	100
Detector technology	CCD
Collection date	2005-04-28
Detector	ADSC QUANTUM 4
Wavelength(s)	0.933
Spacegroup name	P 1 21 1
Unit cell lengths	39.328, 87.214, 40.230
Unit cell angles	90.00, 92.39, 90.00

Refinement procedure

Resolution	27.530 - 1.520
R-factor	0.176
Rwork	0.174
R-free	0.20830
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	In-house SYK model
RMSD bond length	0.011
RMSD bond angle	1.020
Data reduction software	MOSFLM (6.2.4)
Data scaling software	SCALA (3.2.5)
Refinement software	BUSTER (2.11.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	27.535	29.553	1.520
High resolution limit [Å]	1.440	4.550	1.440
Rmerge	0.055	0.036	0.501
Total number of observations	180152	5209	26188
Number of reflections	48894
<I/σ(I)>	13.4	32.1	2.3
Completeness [%]	99.9	96.2	100
Redundancy	3.7	3.4	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	30% Jeffamine ED-2001, 10% Glycerol, 0.1M MES pH6.0, 5mM TCEP