4YJ5
Crystal structure of PKM2 mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL13B1 |
Synchrotron site | NSRRC |
Beamline | BL13B1 |
Temperature [K] | 150 |
Detector technology | CCD |
Collection date | 2013-03-29 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 73.217, 140.887, 108.720 |
Unit cell angles | 90.00, 93.97, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.410 |
R-factor | 0.14791 |
Rwork | 0.144 |
R-free | 0.21731 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4b2d |
RMSD bond length | 0.015 |
RMSD bond angle | 1.906 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | AMoRE |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.500 |
High resolution limit [Å] | 2.400 | 2.410 |
Rmerge | 0.078 | 0.391 |
Number of reflections | 83766 | |
<I/σ(I)> | 17 | 4.18 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 4.3 | 4.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 298 | 0.1 M KCl, 0.2M Na tartrate, 18% PEG3350 |