4YHP
Crystal structure of 309M3-B Fab in complex with H3K9me3 peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-04-19 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 162.773, 159.885, 128.759 |
| Unit cell angles | 90.00, 90.01, 90.00 |
Refinement procedure
| Resolution | 31.380 - 2.530 |
| R-factor | 0.213 |
| Rwork | 0.211 |
| R-free | 0.25000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2r8s 2dd8 1mhp |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.862 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.7.3_928) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.540 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.065 | 0.611 |
| Number of reflections | 112308 | |
| <I/σ(I)> | 20.6 | 2.4 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 292 | 22% PEG 3350, 8% Tacsimate (pH8.0), 3% Xylitol |
