4YHJ
Structure and Function of the Hypertension Variant A486V of G Protein-coupled Receptor Kinase 4 (GRK4)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2009-05-02 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 104.838, 104.838, 221.838 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.000 - 2.600 |
| R-factor | 0.1996 |
| Rwork | 0.197 |
| R-free | 0.24770 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | BUSTER-TNT (BUSTER 2.11.5) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 47.000 |
| High resolution limit [Å] | 2.600 |
| Number of reflections | 44032 |
| <I/σ(I)> | 10.6 |
| Completeness [%] | 99.6 |
| Redundancy | 9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | in 0.1 M sodium citrate, pH 5-6.5, 15-27.5 % (w/v) polyethylene glycol 3350 (PEG3350) |
