4YHC
Crystal structure of the WD40 domain of SCAP from fission yeast
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-04-08 |
Detector | RAYONIX MX325HE |
Wavelength(s) | 1.0000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 38.009, 109.714, 103.454 |
Unit cell angles | 90.00, 100.46, 90.00 |
Refinement procedure
Resolution | 40.000 - 2.050 |
R-factor | 0.1798 |
Rwork | 0.177 |
R-free | 0.22930 |
Structure solution method | SAD |
RMSD bond length | 0.017 |
RMSD bond angle | 1.836 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 40.000 |
High resolution limit [Å] | 2.050 |
Number of reflections | 51974 |
<I/σ(I)> | 27.3 |
Completeness [%] | 99.1 |
Redundancy | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.3 | 291 | 20% (v/v) PEG 3350, 0.2M Na3Citrate |