4YH7
Crystal structure of PTPdelta ectodomain in complex with IL1RAPL1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-06-16 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1.000 |
| Spacegroup name | I 41 |
| Unit cell lengths | 286.295, 286.295, 70.183 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.090 - 4.400 |
| R-factor | 0.292 |
| Rwork | 0.290 |
| R-free | 0.32460 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4YFC 2dju 2dlh |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.575 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL-2000 |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 4.480 |
| High resolution limit [Å] | 4.400 | 4.400 |
| Rmerge | 0.424 | |
| Number of reflections | 17618 | |
| <I/σ(I)> | 17.7 | 2.2 |
| Completeness [%] | 95.6 | 86 |
| Redundancy | 4.2 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 15% PEG4000, 0.1 M MES |
