4YGD
Crystal structure of ERGIC-53/MCFD2, monoclinic calcium-bound form 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2011-07-24 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.90000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 103.063, 58.765, 119.386 |
| Unit cell angles | 90.00, 109.01, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.510 |
| R-factor | 0.195 |
| Rwork | 0.193 |
| R-free | 0.23200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3a4u |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.533 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.540 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.050 | 0.178 |
| Number of reflections | 46251 | |
| <I/σ(I)> | 34.9 | 8.8 |
| Completeness [%] | 99.4 | 97.8 |
| Redundancy | 3.6 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 19% PEG3350, 8% ethylene glycol, 0.1 M Bis-Tris |
