4YG6
Structural basis of glycan recognition in neonate-specific rotaviruses
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-01-24 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.977408 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.200, 140.280, 54.600 |
| Unit cell angles | 90.00, 90.78, 90.00 |
Refinement procedure
| Resolution | 36.200 - 1.460 |
| R-factor | 0.177 |
| Rwork | 0.176 |
| R-free | 0.19700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4yg3 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.100 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE: 1.8.4_1496)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.200 | 1.540 |
| High resolution limit [Å] | 1.460 | 1.460 |
| Rmerge | 0.074 | 0.333 |
| Number of reflections | 93062 | |
| <I/σ(I)> | 11 | 4.2 |
| Completeness [%] | 99.0 | 99.5 |
| Redundancy | 4 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.2 | 293 | 0.1 M Phosphate citrate, 1.6 M Sodium dihydrogen phosphate, 0.4 M di-Potassium hydrogen phosphate. |
