4YDE

CRYSTAL STRUCTURE OF CANDIDA ALBICANS PROTEIN FARNESYLTRANSFERASE BINARY COMPLEX WITH THE ISOPRENOID FARNESYLDIPHOSPHATE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2009-08-13
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.0
Spacegroup name	P 43 2 2
Unit cell lengths	98.874, 98.874, 188.616
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.822 - 2.701
R-factor	0.1972
Rwork	0.195
R-free	0.22810
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4l9p
RMSD bond length	0.003
RMSD bond angle	0.574
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (dev_1839)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.820	2.790
High resolution limit [Å]	2.700	2.700
Rmerge		0.670
Number of reflections	26416
<I/σ(I)>	31.1	4.2
Completeness [%]	100.0	100
Redundancy	14.5	14.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.2	290	9%-13% PEG 2050 chips, 100 mM HEPES pH 7.2 100 -200 mM CaCl2, 5 uM ZnCl2