4YC7
Crystal structure of human FMNL2 GBD-FH3 Domains bound to Cdc42-GppNHp
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-20 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 91.094, 91.094, 144.275 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.400 - 2.500 |
| R-factor | 0.1964 |
| Rwork | 0.194 |
| R-free | 0.25340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB accession code 3EG5 Chain A |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.133 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.400 | |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.053 | 0.336 |
| Number of reflections | 22213 | |
| <I/σ(I)> | 25.9 | 4.4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 14.1 | 14.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 20 mM Tris/HCl (pH 8.0), 14% (v/v) PEG 3350, 0.25 M magnesium acetate |
