4YBQ
Rat GLUT5 with Fv in the outward-open form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-11-22 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 76.773, 151.541, 106.396 |
| Unit cell angles | 90.00, 97.25, 90.00 |
Refinement procedure
| Resolution | 36.491 - 3.270 |
| R-factor | 0.2442 |
| Rwork | 0.242 |
| R-free | 0.28810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3o7q |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.972 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 3.270 |
| Number of reflections | 38017 |
| <I/σ(I)> | 1.22 |
| Completeness [%] | 99.9 |
| Redundancy | 13 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 33-35% PEG400, 0.12 M CaCl2, 0.1 M Tris-HCl pH8.0 |
