4Y6Q

Human SIRT2 in complex with 2-O-myristoyl-ADP-ribose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-12-10
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.98
Spacegroup name	P 1 21 1
Unit cell lengths	61.186, 142.369, 62.626
Unit cell angles	90.00, 94.41, 90.00

Refinement procedure

Resolution	20.000 - 1.900
R-factor	0.233
Rwork	0.231
R-free	0.26700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1j8f 3zgo
RMSD bond length	0.009
RMSD bond angle	1.414
Refinement software	REFMAC (5.8.0103)

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	1.900
Number of reflections	90675
<I/σ(I)>	17
Completeness [%]	98.3
Redundancy	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	PEG 2000