4Y5V

Diabody 305 complex with EpoR

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-02-20
Detector	PSI PILATUS 6M
Wavelength(s)	.9795
Spacegroup name	C 2 2 21
Unit cell lengths	125.730, 215.021, 171.905
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.576 - 2.604
R-factor	0.1888
Rwork	0.187
R-free	0.22880
RMSD bond length	0.002
RMSD bond angle	0.650
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: dev_1839))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.576	2.697
High resolution limit [Å]	2.600	2.604
Rmerge	0.137	0.665
Number of reflections	68355
<I/σ(I)>	13.6	1.7
Completeness [%]	96.0	77.43
Redundancy	6.4	4.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	HEPES, PEG 4000