4Y49
Crystal structure of yeast N-terminal acetyltransferase (ppGpp) NatE in complex with a bisubstrate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-01-30 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97856 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 111.723, 146.486, 254.107 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.630 - 3.950 |
| R-factor | 0.35796 |
| Rwork | 0.355 |
| R-free | 0.42087 |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.869 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.630 | 4.090 |
| High resolution limit [Å] | 3.950 | 3.950 |
| Rmerge | 0.111 | 0.343 |
| Number of reflections | 36307 | |
| <I/σ(I)> | 8.6 | 4.5 |
| Completeness [%] | 97.0 | 83.9 |
| Redundancy | 6.4 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 17% Jeffamine ED-2001 pH 7.0, 0.1 M imidazole, pH 6.2 |
