4Y19

immune complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	110
Detector technology	CCD
Collection date	2013-08-21
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.954
Spacegroup name	I 2 2 2
Unit cell lengths	115.760, 152.740, 153.090
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.600 - 2.500
R-factor	0.1616
Rwork	0.160
R-free	0.19590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4g8f 1bd2 4mdi
RMSD bond length	0.010
RMSD bond angle	1.110
Data reduction software	XDS
Data scaling software	Aimless
Refinement software	BUSTER (2.10.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.600	2.590
High resolution limit [Å]	2.500	2.500
Rmerge	0.137	0.629
Number of reflections	47168
<I/σ(I)>	12.7	3.8
Completeness [%]	100.0	100
Redundancy	8.1	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	100 mM MES pH 6.0, 2 M ammonium sulfate and 0.2 M sodium malonate