4G8F

Crystal Structure of clone42 TCR

Summary for 4G8F

• Entry DOI: 10.2210/pdb4g8f/pdb
• Related: 4G8E 4G8G 4G8I 4G9D 4G9F
• Descriptor: alpha chain clone 42 TCR, beta chain clone 42 TCR (3 entities in total)
• Functional Keywords: tcr, t cell, cd1b, gmm, lipid recognition, immune system
• Biological source: Homo sapiens; More
• Total number of polymer chains: 2
• Total formula weight: 49958.64

Authors

Gras, S.,Bhati, M.,Rossjohn, J. (deposition date: 2012-07-23, release date: 2013-05-08, Last modification date: 2024-11-27)

Primary citation

Van Rhijn, I.,Kasmar, A.,de Jong, A.,Gras, S.,Bhati, M.,Doorenspleet, M.E.,de Vries, N.,Godfrey, D.I.,Altman, J.D.,de Jager, W.,Rossjohn, J.,Moody, D.B.
A conserved human T cell population targets mycobacterial antigens presented by CD1b.
Nat.Immunol., 14:706-713, 2013

PubMed Abstract: Human T cell antigen receptors (TCRs) pair in millions of combinations to create complex and unique T cell repertoires for each person. Through the use of tetramers to analyze TCRs reactive to the antigen-presenting molecule CD1b, we detected T cells with highly stereotyped TCR α-chains present among genetically unrelated patients with tuberculosis. The germline-encoded, mycolyl lipid-reactive (GEM) TCRs had an α-chain bearing the variable (V) region TRAV1-2 rearranged to the joining (J) region TRAJ9 with few nontemplated (N)-region additions. Analysis of TCRs by high-throughput sequencing, binding and crystallography showed linkage of TCRα sequence motifs to high-affinity recognition of antigen. Thus, the CD1-reactive TCR repertoire is composed of at least two compartments: high-affinity GEM TCRs, and more-diverse TCRs with low affinity for CD1b-lipid complexes. We found high interdonor conservation of TCRs that probably resulted from selection by a nonpolymorphic antigen-presenting molecule and an immunodominant antigen.

PubMed: 23727893
DOI: 10.1038/ni.2630

Experimental method

X-RAY DIFFRACTION (2.1 Å)