4XXO
Crystal Structure of Human APOBEC3A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-05-24 |
| Detector | RIGAKU SATURN 944 |
| Wavelength(s) | 1.541800 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 94.752, 94.752, 213.962 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.731 - 2.843 |
| R-factor | 0.2166 |
| Rwork | 0.214 |
| R-free | 0.25310 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3v4k |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.664 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.731 | 2.944 |
| High resolution limit [Å] | 2.840 | 2.843 |
| Rmerge | 0.326 | 3.924 |
| Number of reflections | 13996 | |
| <I/σ(I)> | 7.82 | 0.81 |
| Completeness [%] | 99.8 | 99.27 |
| Redundancy | 11 | 11.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 277 | diamond shaped crystals of 50 uM x 20 uM grew after 3 years of incubation |
