4XXK

Crystal structure of the Semet-derivative of the Bilin-binding domain of phycobilisome core-membrane linker ApcE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-05-10
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.979160
Spacegroup name	P 1 21 1
Unit cell lengths	36.980, 66.590, 81.420
Unit cell angles	90.00, 91.07, 90.00

Refinement procedure

Resolution	19.487 - 2.970
R-factor	0.1817
Rwork	0.176
R-free	0.25210
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.279
Data scaling software	XSCALE
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.700	3.130
High resolution limit [Å]	2.970	2.970
Number of reflections	8228
<I/σ(I)>	21.7	12.9
Completeness [%]	99.3	97.4
Redundancy	6.8	6.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH		285	16% up to 26% (w/v) PEG 3350, 0.25 M NaSCN