4XWW

Crystal structure of RNase J complexed with RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	95
Detector technology	CCD
Collection date	2012-10-30
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	67.120, 87.860, 249.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.591 - 1.700
R-factor	0.196
Rwork	0.195
R-free	0.21700
RMSD bond length	0.007
RMSD bond angle	1.109
Data reduction software	XSCALE
Phasing software	XDS
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000		1.740
High resolution limit [Å]	1.700	7.600	1.700
Rmerge	0.102	0.081	0.697
Rmeas	0.112	0.091	0.796
Total number of observations	936565
Number of reflections	158299	1474	9536
<I/σ(I)>	10.69	16.42	1.92
Completeness [%]	97.2	72.2	79.8
Redundancy	5.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	PEG 4000, MnCl2, MES