4XWO
Structure of Get3 bound to the transmembrane domain of Sec22
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-03-10 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9795 |
Spacegroup name | P 1 |
Unit cell lengths | 112.580, 119.450, 147.570 |
Unit cell angles | 71.89, 89.86, 66.61 |
Refinement procedure
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.723 | 2.850 |
High resolution limit [Å] | 2.750 | 2.750 |
Number of reflections | 157053 | |
<I/σ(I)> | 8.2 | 1.4 |
Completeness [%] | 91.0 | 89 |
Redundancy | 1.7 | 1.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 13% PEG 3350, 25 mM succinic acid pH 7.0 |