Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

4XWO

Structure of Get3 bound to the transmembrane domain of Sec22

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000750	biological_process	pheromone-dependent signal transduction involved in conjugation with cellular fusion
A	0005085	molecular_function	guanyl-nucleotide exchange factor activity
A	0005515	molecular_function	protein binding
A	0005524	molecular_function	ATP binding
A	0005737	cellular_component	cytoplasm
A	0005783	cellular_component	endoplasmic reticulum
A	0005789	cellular_component	endoplasmic reticulum membrane
A	0005794	cellular_component	Golgi apparatus
A	0005829	cellular_component	cytosol
A	0006457	biological_process	protein folding
A	0006620	biological_process	post-translational protein targeting to endoplasmic reticulum membrane
A	0006890	biological_process	retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum
A	0008270	molecular_function	zinc ion binding
A	0009408	biological_process	response to heat
A	0010038	biological_process	response to metal ion
A	0016192	biological_process	vesicle-mediated transport
A	0016787	molecular_function	hydrolase activity
A	0016887	molecular_function	ATP hydrolysis activity
A	0034599	biological_process	cellular response to oxidative stress
A	0042802	molecular_function	identical protein binding
A	0043529	cellular_component	GET complex
A	0044183	molecular_function	protein folding chaperone
A	0045048	biological_process	protein insertion into ER membrane
A	0046685	biological_process	response to arsenic-containing substance
A	0046872	molecular_function	metal ion binding
A	0051082	molecular_function	unfolded protein binding
A	0071816	biological_process	tail-anchored membrane protein insertion into ER membrane
B	0000750	biological_process	pheromone-dependent signal transduction involved in conjugation with cellular fusion
B	0005085	molecular_function	guanyl-nucleotide exchange factor activity
B	0005515	molecular_function	protein binding
B	0005524	molecular_function	ATP binding
B	0005737	cellular_component	cytoplasm
B	0005783	cellular_component	endoplasmic reticulum
B	0005789	cellular_component	endoplasmic reticulum membrane
B	0005794	cellular_component	Golgi apparatus
B	0005829	cellular_component	cytosol
B	0006457	biological_process	protein folding
B	0006620	biological_process	post-translational protein targeting to endoplasmic reticulum membrane
B	0006890	biological_process	retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum
B	0008270	molecular_function	zinc ion binding
B	0009408	biological_process	response to heat
B	0010038	biological_process	response to metal ion
B	0016192	biological_process	vesicle-mediated transport
B	0016787	molecular_function	hydrolase activity
B	0016887	molecular_function	ATP hydrolysis activity
B	0034599	biological_process	cellular response to oxidative stress
B	0042802	molecular_function	identical protein binding
B	0043529	cellular_component	GET complex
B	0044183	molecular_function	protein folding chaperone
B	0045048	biological_process	protein insertion into ER membrane
B	0046685	biological_process	response to arsenic-containing substance
B	0046872	molecular_function	metal ion binding
B	0051082	molecular_function	unfolded protein binding
B	0071816	biological_process	tail-anchored membrane protein insertion into ER membrane
G	0000750	biological_process	pheromone-dependent signal transduction involved in conjugation with cellular fusion
G	0005085	molecular_function	guanyl-nucleotide exchange factor activity
G	0005515	molecular_function	protein binding
G	0005524	molecular_function	ATP binding
G	0005737	cellular_component	cytoplasm
G	0005783	cellular_component	endoplasmic reticulum
G	0005789	cellular_component	endoplasmic reticulum membrane
G	0005794	cellular_component	Golgi apparatus
G	0005829	cellular_component	cytosol
G	0006457	biological_process	protein folding
G	0006620	biological_process	post-translational protein targeting to endoplasmic reticulum membrane
G	0006890	biological_process	retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum
G	0008270	molecular_function	zinc ion binding
G	0009408	biological_process	response to heat
G	0010038	biological_process	response to metal ion
G	0016192	biological_process	vesicle-mediated transport
G	0016787	molecular_function	hydrolase activity
G	0016887	molecular_function	ATP hydrolysis activity
G	0034599	biological_process	cellular response to oxidative stress
G	0042802	molecular_function	identical protein binding
G	0043529	cellular_component	GET complex
G	0044183	molecular_function	protein folding chaperone
G	0045048	biological_process	protein insertion into ER membrane
G	0046685	biological_process	response to arsenic-containing substance
G	0046872	molecular_function	metal ion binding
G	0051082	molecular_function	unfolded protein binding
G	0071816	biological_process	tail-anchored membrane protein insertion into ER membrane
H	0000750	biological_process	pheromone-dependent signal transduction involved in conjugation with cellular fusion
H	0005085	molecular_function	guanyl-nucleotide exchange factor activity
H	0005515	molecular_function	protein binding
H	0005524	molecular_function	ATP binding
H	0005737	cellular_component	cytoplasm
H	0005783	cellular_component	endoplasmic reticulum
H	0005789	cellular_component	endoplasmic reticulum membrane
H	0005794	cellular_component	Golgi apparatus
H	0005829	cellular_component	cytosol
H	0006457	biological_process	protein folding
H	0006620	biological_process	post-translational protein targeting to endoplasmic reticulum membrane
H	0006890	biological_process	retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum
H	0008270	molecular_function	zinc ion binding
H	0009408	biological_process	response to heat
H	0010038	biological_process	response to metal ion
H	0016192	biological_process	vesicle-mediated transport
H	0016787	molecular_function	hydrolase activity
H	0016887	molecular_function	ATP hydrolysis activity
H	0034599	biological_process	cellular response to oxidative stress
H	0042802	molecular_function	identical protein binding
H	0043529	cellular_component	GET complex
H	0044183	molecular_function	protein folding chaperone
H	0045048	biological_process	protein insertion into ER membrane
H	0046685	biological_process	response to arsenic-containing substance
H	0046872	molecular_function	metal ion binding
H	0051082	molecular_function	unfolded protein binding
H	0071816	biological_process	tail-anchored membrane protein insertion into ER membrane
M	0000750	biological_process	pheromone-dependent signal transduction involved in conjugation with cellular fusion
M	0005085	molecular_function	guanyl-nucleotide exchange factor activity
M	0005515	molecular_function	protein binding
M	0005524	molecular_function	ATP binding
M	0005737	cellular_component	cytoplasm
M	0005783	cellular_component	endoplasmic reticulum
M	0005789	cellular_component	endoplasmic reticulum membrane
M	0005794	cellular_component	Golgi apparatus
M	0005829	cellular_component	cytosol
M	0006457	biological_process	protein folding
M	0006620	biological_process	post-translational protein targeting to endoplasmic reticulum membrane
M	0006890	biological_process	retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum
M	0008270	molecular_function	zinc ion binding
M	0009408	biological_process	response to heat
M	0010038	biological_process	response to metal ion
M	0016192	biological_process	vesicle-mediated transport
M	0016787	molecular_function	hydrolase activity
M	0016887	molecular_function	ATP hydrolysis activity
M	0034599	biological_process	cellular response to oxidative stress
M	0042802	molecular_function	identical protein binding
M	0043529	cellular_component	GET complex
M	0044183	molecular_function	protein folding chaperone
M	0045048	biological_process	protein insertion into ER membrane
M	0046685	biological_process	response to arsenic-containing substance
M	0046872	molecular_function	metal ion binding
M	0051082	molecular_function	unfolded protein binding
M	0071816	biological_process	tail-anchored membrane protein insertion into ER membrane
N	0000750	biological_process	pheromone-dependent signal transduction involved in conjugation with cellular fusion
N	0005085	molecular_function	guanyl-nucleotide exchange factor activity
N	0005515	molecular_function	protein binding
N	0005524	molecular_function	ATP binding
N	0005737	cellular_component	cytoplasm
N	0005783	cellular_component	endoplasmic reticulum
N	0005789	cellular_component	endoplasmic reticulum membrane
N	0005794	cellular_component	Golgi apparatus
N	0005829	cellular_component	cytosol
N	0006457	biological_process	protein folding
N	0006620	biological_process	post-translational protein targeting to endoplasmic reticulum membrane
N	0006890	biological_process	retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum
N	0008270	molecular_function	zinc ion binding
N	0009408	biological_process	response to heat
N	0010038	biological_process	response to metal ion
N	0016192	biological_process	vesicle-mediated transport
N	0016787	molecular_function	hydrolase activity
N	0016887	molecular_function	ATP hydrolysis activity
N	0034599	biological_process	cellular response to oxidative stress
N	0042802	molecular_function	identical protein binding
N	0043529	cellular_component	GET complex
N	0044183	molecular_function	protein folding chaperone
N	0045048	biological_process	protein insertion into ER membrane
N	0046685	biological_process	response to arsenic-containing substance
N	0046872	molecular_function	metal ion binding
N	0051082	molecular_function	unfolded protein binding
N	0071816	biological_process	tail-anchored membrane protein insertion into ER membrane
S	0000750	biological_process	pheromone-dependent signal transduction involved in conjugation with cellular fusion
S	0005085	molecular_function	guanyl-nucleotide exchange factor activity
S	0005515	molecular_function	protein binding
S	0005524	molecular_function	ATP binding
S	0005737	cellular_component	cytoplasm
S	0005783	cellular_component	endoplasmic reticulum
S	0005789	cellular_component	endoplasmic reticulum membrane
S	0005794	cellular_component	Golgi apparatus
S	0005829	cellular_component	cytosol
S	0006457	biological_process	protein folding
S	0006620	biological_process	post-translational protein targeting to endoplasmic reticulum membrane
S	0006890	biological_process	retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum
S	0008270	molecular_function	zinc ion binding
S	0009408	biological_process	response to heat
S	0010038	biological_process	response to metal ion
S	0016192	biological_process	vesicle-mediated transport
S	0016787	molecular_function	hydrolase activity
S	0016887	molecular_function	ATP hydrolysis activity
S	0034599	biological_process	cellular response to oxidative stress
S	0042802	molecular_function	identical protein binding
S	0043529	cellular_component	GET complex
S	0044183	molecular_function	protein folding chaperone
S	0045048	biological_process	protein insertion into ER membrane
S	0046685	biological_process	response to arsenic-containing substance
S	0046872	molecular_function	metal ion binding
S	0051082	molecular_function	unfolded protein binding
S	0071816	biological_process	tail-anchored membrane protein insertion into ER membrane
T	0000750	biological_process	pheromone-dependent signal transduction involved in conjugation with cellular fusion
T	0005085	molecular_function	guanyl-nucleotide exchange factor activity
T	0005515	molecular_function	protein binding
T	0005524	molecular_function	ATP binding
T	0005737	cellular_component	cytoplasm
T	0005783	cellular_component	endoplasmic reticulum
T	0005789	cellular_component	endoplasmic reticulum membrane
T	0005794	cellular_component	Golgi apparatus
T	0005829	cellular_component	cytosol
T	0006457	biological_process	protein folding
T	0006620	biological_process	post-translational protein targeting to endoplasmic reticulum membrane
T	0006890	biological_process	retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum
T	0008270	molecular_function	zinc ion binding
T	0009408	biological_process	response to heat
T	0010038	biological_process	response to metal ion
T	0016192	biological_process	vesicle-mediated transport
T	0016787	molecular_function	hydrolase activity
T	0016887	molecular_function	ATP hydrolysis activity
T	0034599	biological_process	cellular response to oxidative stress
T	0042802	molecular_function	identical protein binding
T	0043529	cellular_component	GET complex
T	0044183	molecular_function	protein folding chaperone
T	0045048	biological_process	protein insertion into ER membrane
T	0046685	biological_process	response to arsenic-containing substance
T	0046872	molecular_function	metal ion binding
T	0051082	molecular_function	unfolded protein binding
T	0071816	biological_process	tail-anchored membrane protein insertion into ER membrane

Functional Information from PDB Data

site_id	AC1
Number of Residues	6
Details	binding site for residue MG A 401

Chain	Residue
A	THR32
A	ADP403
A	ATP404
A	HOH517
A	HOH518
A	HOH519

site_id	AC2
Number of Residues	4
Details	binding site for residue ZN A 402

Chain	Residue
B	CYS288
A	CYS285
A	CYS288
B	CYS285

site_id	AC3
Number of Residues	18
Details	binding site for residue ADP A 403

Chain	Residue
A	GLY28
A	VAL29
A	GLY30
A	LYS31
A	THR32
A	THR33
A	ASN272
A	PRO315
A	LEU316
A	CYS317
A	ILE321
A	PHE330
A	MG401
A	ATP404
A	HOH517
B	LYS26
B	GLU245
B	PHE246

site_id	AC4
Number of Residues	22
Details	binding site for residue ATP A 404

Chain	Residue
A	GLY27
A	GLY28
A	GLY30
A	LYS31
A	THR32
A	THR33
A	ASN57
A	ASN272
A	PRO315
A	LEU316
A	CYS317
A	ILE321
A	PHE330
A	MG401
A	ADP403
A	HOH517
A	HOH518
B	LYS26
B	GLY27
B	GLU245
B	PHE246
B	LEU247

site_id	AC5
Number of Residues	6
Details	binding site for residue MG B 401

Chain	Residue
B	THR32
B	ADP402
B	ATP403
B	HOH514
B	HOH515
B	HOH516

site_id	AC6
Number of Residues	17
Details	binding site for residue ADP B 402

Chain	Residue
A	LYS26
A	GLU245
B	GLY28
B	GLY30
B	LYS31
B	THR32
B	THR33
B	ASN272
B	PRO315
B	LEU316
B	CYS317
B	ILE321
B	PHE330
B	MG401
B	ATP403
B	HOH514
B	HOH515

site_id	AC7
Number of Residues	22
Details	binding site for residue ATP B 403

Chain	Residue
A	LYS26
A	GLY27
A	GLU245
A	LEU247
B	GLY27
B	GLY28
B	GLY30
B	LYS31
B	THR32
B	THR33
B	ASN57
B	PRO169
B	ASN272
B	PRO315
B	LEU316
B	CYS317
B	ILE321
B	PHE330
B	MG401
B	ADP402
B	HOH514
B	HOH515

site_id	AC8
Number of Residues	6
Details	binding site for residue MG G 401

Chain	Residue
G	HOH517
G	HOH518
G	THR32
G	ADP403
G	ATP404
G	HOH516

site_id	AC9
Number of Residues	4
Details	binding site for residue ZN G 402

Chain	Residue
G	CYS285
G	CYS288
H	CYS285
H	CYS288

site_id	AD1
Number of Residues	18
Details	binding site for residue ADP G 403

Chain	Residue
G	GLY28
G	VAL29
G	GLY30
G	LYS31
G	THR32
G	THR33
G	ASN272
G	PRO315
G	LEU316
G	CYS317
G	ILE321
G	PHE330
G	MG401
G	ATP404
G	HOH516
G	HOH517
H	LYS26
H	GLU245

site_id	AD2
Number of Residues	22
Details	binding site for residue ATP G 404

Chain	Residue
G	GLY27
G	GLY28
G	GLY30
G	LYS31
G	THR32
G	THR33
G	ASN57
G	ASN272
G	PRO315
G	LEU316
G	CYS317
G	ILE321
G	PHE330
G	MG401
G	ADP403
G	HOH516
G	HOH517
G	HOH518
H	LYS26
H	GLY27
H	GLU245
H	LEU247

site_id	AD3
Number of Residues	6
Details	binding site for residue MG H 401

Chain	Residue
H	THR32
H	ADP402
H	ATP403
H	HOH519
H	HOH520
H	HOH521

site_id	AD4
Number of Residues	23
Details	binding site for residue ATP H 403

Chain	Residue
G	LYS26
G	GLY27
G	GLU245
G	PHE246
G	LEU247
H	GLY27
H	GLY28
H	VAL29
H	GLY30
H	LYS31
H	THR32
H	THR33
H	ASN57
H	PRO169
H	ASN272
H	PRO315
H	LEU316
H	CYS317
H	PHE330
H	MG401
H	ADP402
H	HOH519
H	HOH520

site_id	AD5
Number of Residues	6
Details	binding site for residue MG M 401

Chain	Residue
M	THR32
M	ADP403
M	ATP404
M	HOH515
M	HOH516
M	HOH520

site_id	AD6
Number of Residues	4
Details	binding site for residue ZN M 402

Chain	Residue
M	CYS285
M	CYS288
N	CYS285
N	CYS288

site_id	AD7
Number of Residues	6
Details	binding site for residue MG N 401

Chain	Residue
N	THR32
N	ADP402
N	ATP403
N	HOH520
N	HOH521
N	HOH522

site_id	AD8
Number of Residues	15
Details	binding site for residue ADP N 402

Chain	Residue
M	LYS26
M	GLU245
N	GLY28
N	GLY30
N	LYS31
N	THR32
N	THR33
N	ASN272
N	PRO315
N	CYS317
N	ILE321
N	PHE330
N	MG401
N	ATP403
N	HOH520

site_id	AD9
Number of Residues	21
Details	binding site for residue ATP N 403

Chain	Residue
M	LYS26
M	GLY27
M	GLU245
M	LEU247
N	GLY27
N	GLY28
N	GLY30
N	LYS31
N	THR32
N	THR33
N	ASN57
N	ASN272
N	PRO315
N	CYS317
N	ILE321
N	PHE330
N	MG401
N	ADP402
N	HOH520
N	HOH521
N	HOH522

site_id	AE1
Number of Residues	6
Details	binding site for residue MG S 401

Chain	Residue
S	THR32
S	ADP403
S	ATP404
S	HOH513
S	HOH514
S	HOH516

site_id	AE2
Number of Residues	4
Details	binding site for residue ZN S 402

Chain	Residue
S	CYS285
S	CYS288
T	CYS285
T	CYS288

site_id	AE3
Number of Residues	20
Details	binding site for residue ADP S 403

Chain	Residue
S	GLY28
S	GLY30
S	LYS31
S	THR32
S	THR33
S	ASN272
S	PRO315
S	LEU316
S	CYS317
S	GLY319
S	ILE321
S	PHE330
S	MG401
S	ATP404
S	HOH513
S	HOH516
T	LYS26
T	GLU245
T	LEU247
T	ARG291

site_id	AE4
Number of Residues	23
Details	binding site for residue ATP S 404

Chain	Residue
S	GLY27
S	GLY28
S	GLY30
S	LYS31
S	THR32
S	THR33
S	ASN57
S	ASN272
S	PRO315
S	LEU316
S	CYS317
S	ILE321
S	PHE330
S	MG401
S	ADP403
S	HOH513
S	HOH514
S	HOH516
T	LYS26
T	GLY27
T	GLU245
T	LEU247
T	ARG291

site_id	AE5
Number of Residues	7
Details	binding site for residue MG T 401

Chain	Residue
T	THR32
T	ASN61
T	ADP402
T	ATP403
T	HOH519
T	HOH520
T	HOH521

site_id	AE6
Number of Residues	25
Details	binding site for residue ADP H 402

Chain	Residue
G	LYS26
G	GLU245
G	PHE246
G	LEU247
H	VAL23
H	GLY25
H	LYS26
H	GLY27
H	GLY28
H	GLY30
H	THR32
H	THR33
H	SER34
H	SER35
H	ASP166
H	ASN272
H	PRO315
H	LEU316
H	CYS317
H	ILE321
H	PHE330
H	MG401
H	ATP403
H	HOH519
H	HOH520

site_id	AE7
Number of Residues	18
Details	binding site for residue ADP M 403

Chain	Residue
M	GLY28
M	GLY30
M	LYS31
M	THR32
M	THR33
M	ASN272
M	LEU274
M	MET314
M	LEU316
M	CYS317
M	ILE321
M	PHE330
M	MG401
M	ATP404
M	HOH520
N	LYS26
N	GLU245
N	LEU247

site_id	AE8
Number of Residues	24
Details	binding site for residue ATP M 404

Chain	Residue
M	GLY27
M	GLY28
M	VAL29
M	GLY30
M	LYS31
M	THR32
M	THR33
M	ASN57
M	PRO169
M	ASN272
M	LEU274
M	MET314
M	LEU316
M	CYS317
M	ILE321
M	PHE330
M	MG401
M	ADP403
M	HOH515
M	HOH516
M	HOH520
N	LYS26
N	GLU245
N	LEU247

site_id	AE9
Number of Residues	23
Details	binding site for residue ADP T 402

Chain	Residue
S	LYS26
S	GLU245
T	VAL23
T	GLY24
T	GLY25
T	LYS26
T	GLY27
T	GLY28
T	GLY30
T	THR32
T	THR33
T	SER34
T	SER35
T	THR167
T	ASN272
T	PRO315
T	LEU316
T	CYS317
T	ILE321
T	PHE330
T	MG401
T	ATP403
T	HOH519

site_id	AF1
Number of Residues	25
Details	binding site for residue ATP T 403

Chain	Residue
S	LYS26
S	GLY27
S	GLU245
S	LEU247
T	VAL23
T	GLY24
T	GLY25
T	LYS26
T	GLY27
T	GLY28
T	GLY30
T	THR32
T	THR33
T	SER34
T	SER35
T	THR167
T	ASN272
T	PRO315
T	LEU316
T	CYS317
T	ILE321
T	PHE330
T	MG401
T	ADP402
T	HOH519

Functional Information from PROSITE/UniProt

site_id	PS00290
Number of Residues	7
Details	IG_MHC Immunoglobulins and major histocompatibility complex proteins signature. YACEVTH

Chain	Residue	Details
D	TYR195-HIS201
C	TYR203-HIS209

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	8
Details	ACT_SITE:

Chain	Residue	Details
A	ASN57
B	ASN57
G	ASN57
H	ASN57
M	ASN57
N	ASN57
S	ASN57
T	ASN57

site_id	SWS_FT_FI2
Number of Residues	32
Details	BINDING:

Chain	Residue	Details
A	LYS26
G	GLU245
G	ASN272
G	PRO315
H	LYS26
H	GLU245
H	ASN272
H	PRO315
M	LYS26
M	GLU245
M	ASN272
A	GLU245
M	PRO315
N	LYS26
N	GLU245
N	ASN272
N	PRO315
S	LYS26
S	GLU245
S	ASN272
S	PRO315
T	LYS26
A	ASN272
T	GLU245
T	ASN272
T	PRO315
A	PRO315
B	LYS26
B	GLU245
B	ASN272
B	PRO315
G	LYS26

site_id	SWS_FT_FI3
Number of Residues	16
Details	BINDING: BINDING => ECO:0000255\|HAMAP-Rule:MF_03112, ECO:0000269\|PubMed:19675567

Chain	Residue	Details
A	CYS285
M	CYS288
N	CYS285
N	CYS288
S	CYS285
S	CYS288
T	CYS285
T	CYS288
A	CYS288
B	CYS285
B	CYS288
G	CYS285
G	CYS288
H	CYS285
H	CYS288
M	CYS285

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)