4XWG
Crystal Structure of LCAT (C31Y) in complex with Fab1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-05-07 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | H 3 |
| Unit cell lengths | 168.590, 168.590, 93.570 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.005 - 2.650 |
| R-factor | 0.1798 |
| Rwork | 0.176 |
| R-free | 0.25120 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.540 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 84.295 | 39.390 | 2.790 |
| High resolution limit [Å] | 2.650 | 8.380 | 2.650 |
| Rmerge | 0.049 | 0.644 | |
| Rmeas | 0.102 | ||
| Rpim | 0.049 | 0.028 | 0.369 |
| Total number of observations | 119076 | 3671 | 17105 |
| Number of reflections | 28802 | ||
| <I/σ(I)> | 10.3 | 23.1 | 2.2 |
| Completeness [%] | 100.0 | 99 | 100 |
| Redundancy | 4.1 | 4.1 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M sodium acetate, 0.2 M zinc acetate, 0.01 M cobalt chloride, 4-8% PEG 1500 |
