4XT3
Structure of a viral GPCR bound to human chemokine CX3CL1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-04-20 |
| Detector | PILATUS3 6M |
| Wavelength(s) | 1.033 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 59.900, 192.700, 94.400 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.920 - 3.801 |
| R-factor | 0.2878 |
| Rwork | 0.283 |
| R-free | 0.32210 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4mbs 1f2l 3ona |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.874 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: dev_1839)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.920 | 3.937 |
| High resolution limit [Å] | 3.800 | 3.800 |
| Rmerge | 0.275 | 0.555 |
| Number of reflections | 4834 | |
| <I/σ(I)> | 4.1 | 1.6 |
| Completeness [%] | 85.0 | 55.5 |
| Redundancy | 5.6 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 7.4 | 293 | PEG 300, HEPES, ammonium phosphate |
