4XRS
Heterodimeric complex of transcription factors MEIS1 and DLX3 on specific DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-06-14 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9724 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 69.636, 69.845, 116.888 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.314 - 3.500 |
| R-factor | 0.3427 |
| Rwork | 0.331 |
| R-free | 0.35910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3k2a 2djn |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.179 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (phenix.refine: 1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.070 |
| High resolution limit [Å] | 2.900 | 2.964 |
| Rmerge | 0.061 | 0.061 |
| Number of reflections | 11191 | |
| <I/σ(I)> | 13.08 | 0.63 |
| Completeness [%] | 90.5 | 88.39 |
| Redundancy | 4.1 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | PEG 8000, TRIS, Magnesium chloride, buthanol |
