4XRM
homodimer of TALE type homeobox transcription factor MEIS1 complexes with specific DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-01-22 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9724 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 70.620, 79.280, 98.970 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.830 - 1.600 |
| R-factor | 0.1773 |
| Rwork | 0.177 |
| R-free | 0.21080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3k2a |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.790 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.657 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.041 | 0.713 |
| Number of reflections | 36011 | |
| <I/σ(I)> | 14.84 | 1.56 |
| Completeness [%] | 97.3 | 93.87 |
| Redundancy | 4.1 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | PME 5000, Tris, PEG 400, magnesium chloride |
