4XRL
Crystal structure at room temperature of Erk2 in complex with an inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2014-04-14 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.542 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.479, 70.786, 60.489 |
| Unit cell angles | 90.00, 109.48, 90.00 |
Refinement procedure
| Resolution | 30.072 - 2.554 |
| R-factor | 0.162 |
| Rwork | 0.160 |
| R-free | 0.22860 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.214 |
| Data scaling software | SCALA |
| Phasing software | REFMAC |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.070 |
| High resolution limit [Å] | 2.550 |
| Number of reflections | 10787 |
| <I/σ(I)> | 7.6 |
| Completeness [%] | 88.2 |
| Redundancy | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | MES 100mM pH 6.5, (NH4)2SO4 200mM, beta-mercaptoethanol 20mM and 26% (w/v) PEG MME2000 |
