4XRE
Crystal structure of Gnk2 complexed with mannose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NE3A |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2011-11-26 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 143.400, 143.400, 143.400 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.886 - 2.597 |
| R-factor | 0.2511 |
| Rwork | 0.249 |
| R-free | 0.28850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3a2e |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.132 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.640 |
| High resolution limit [Å] | 2.597 | 2.600 |
| Number of reflections | 30598 | |
| <I/σ(I)> | 74.7 | 9.9 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 21.5 | 20.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 28% PEG MME 2000, 0.1M TRIS-HCL, 0.2M AMMONIUM SULFATE |
