4XQ8

Human DNA polymerase lambda- MgdATP binary complex and complex with 6 paired DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL13B1
Synchrotron site	NSRRC
Beamline	BL13B1
Temperature [K]	95
Detector technology	CCD
Collection date	2013-10-22
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0
Spacegroup name	P 65 2 2
Unit cell lengths	83.743, 83.743, 398.746
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	27.411 - 2.798
R-factor	0.187
Rwork	0.182
R-free	0.23990
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4W5D
RMSD bond length	0.009
RMSD bond angle	1.183
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.900
High resolution limit [Å]	2.798	6.020	2.800
Rmerge	0.084	0.026	0.491
Rmeas	0.089	0.028	0.526
Rpim	0.031	0.010	0.187
Total number of observations	178549
Number of reflections	21679
<I/σ(I)>	9.5
Completeness [%]	100.0	99.7	100
Redundancy	8.2	7.4	7.8
CC(1/2)		1.000	0.902

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		277	0.1 M Lithium Sulfate monohydrate, 0.1 M HEPES-Sodium, 0.1 M Potassium Sodium Tartrate