4XPN

Crystal Structure of Protein Phosphate 1 complexed with PP1 binding domain of GADD34

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-04-24
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.98
Spacegroup name	P 21 21 21
Unit cell lengths	64.311, 112.853, 125.013
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.428 - 2.285
R-factor	0.1613
Rwork	0.159
R-free	0.20500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3e7a
RMSD bond length	0.011
RMSD bond angle	1.007
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER (2.5.3)
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.210
High resolution limit [Å]	2.170	5.890	2.170
Rmerge	0.108	0.073
Total number of observations	307472
Number of reflections	49300
<I/σ(I)>	4.8
Completeness [%]	99.8	99.6	99.3
Redundancy	6.2	5.8	4.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.8	277	0.2 M Ammonium phosphate dibasic, 20% w/v Polyethylene glycol 3,350