4XJ5

Crystal structure of Vibrio cholerae DncV 3'-deoxy GTP bound form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	CCD
Collection date	2014-01-19
Detector	ADSC QUANTUM 315r
Wavelength(s)	1
Spacegroup name	C 1 2 1
Unit cell lengths	117.950, 50.060, 72.270
Unit cell angles	90.00, 93.40, 90.00

Refinement procedure

Resolution	46.997 - 1.552
R-factor	0.1757
Rwork	0.175
R-free	0.19330
Structure solution method	SAD
Starting model (for MR)	4xj1
RMSD bond length	0.012
RMSD bond angle	1.338
Data reduction software	XDS
Phasing software	MOLREP
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.550
Number of reflections	60205
<I/σ(I)>	17.6
Completeness [%]	98.5
Redundancy	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	PEG3350, sodium fluoride, Bis-Tris propane