4XI5

gHgL of varicella-zoster virus in complex with human neutralizing antibodies

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-06-21
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.00000
Spacegroup name	C 2 2 21
Unit cell lengths	102.002, 280.552, 175.302
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.322 - 3.900
R-factor	0.2467
Rwork	0.245
R-free	0.27640
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4xhj
RMSD bond length	0.003
RMSD bond angle	0.944
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.1_1168))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	200.000	3.970
High resolution limit [Å]	3.885	3.885
Rmerge	0.067	0.753
Number of reflections	23170
<I/σ(I)>	24.4	1.7
Completeness [%]	99.1
Redundancy	6.3	5.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	0.1M MES pH 6.0 and 4% PEG 4000