4XG2

Crystal structure of ligand-free Syk

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	CCD
Collection date	2010-05-27
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0000
Spacegroup name	P 1 21 1
Unit cell lengths	40.189, 84.799, 41.406
Unit cell angles	90.00, 98.74, 90.00

Refinement procedure

Resolution	42.400 - 2.210
R-factor	0.20427
Rwork	0.203
R-free	0.22681
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1xba
RMSD bond length	0.007
RMSD bond angle	1.124
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0103)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.240
High resolution limit [Å]	2.200	2.200
Number of reflections	13725
<I/σ(I)>	40.79
Completeness [%]	99.1
Redundancy	7.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	278	10~20%(v/v) PEG3350, 100 mM Tris-HCl