4XFS
Structure of IL-18 SER Mutant I
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-03-10 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97926 |
| Spacegroup name | P 1 |
| Unit cell lengths | 32.982, 42.258, 52.343 |
| Unit cell angles | 77.80, 83.63, 67.13 |
Refinement procedure
| Resolution | 50.000 - 1.910 |
| R-factor | 0.18073 |
| Rwork | 0.178 |
| R-free | 0.22871 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3f62 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.377 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.490 | |
| Number of reflections | 18429 | |
| <I/σ(I)> | 19.8 | 2.1 |
| Completeness [%] | 94.6 | 81.6 |
| Redundancy | 4.5 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 47% PEG 2K MME, 0.1M HEPES, 5% DMSO |
