4XF2
Tetragonal structure of Arp2/3 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-01-15 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1 |
| Spacegroup name | P 41 |
| Unit cell lengths | 149.930, 149.930, 265.911 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.536 - 5.000 |
| R-factor | 0.2762 |
| Rwork | 0.275 |
| R-free | 0.30310 |
| Structure solution method | MIR |
| Starting model (for MR) | 1U2V with the calcium and ADP ligands removed |
| RMSD bond length | 0.011 |
| RMSD bond angle | 2.365 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 |
| High resolution limit [Å] | 5.000 | 5.000 |
| Number of reflections | 24560 | |
| <I/σ(I)> | 20.3 | 2.3 |
| Completeness [%] | 96.8 | 84.6 |
| Redundancy | 6.4 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 100 mM Tris 7.0, 15% ethanol |
