4XBM
X-ray crystal structure of Notch ligand Delta-like 1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-04-30 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9537 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 108.150, 118.870, 134.810 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 67.300 - 3.200 |
R-factor | 0.2601 |
Rwork | 0.258 |
R-free | 0.28350 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4cbz |
RMSD bond length | 0.004 |
RMSD bond angle | 0.928 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 67.400 | 3.314 |
High resolution limit [Å] | 3.200 | 3.200 |
Rmerge | 0.432 | |
Number of reflections | 29271 | |
<I/σ(I)> | 6.27 | |
Completeness [%] | 99.9 | |
Redundancy | 7.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.4M potassium thiocyanate 16% PEG3350 0.1M Bis-tris propane pH 7.5 5mM CaCl2 |