4XB2

Hyperthermophilic archaeal homoserine dehydrogenase mutant in complex with NADPH

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2014-08-28
Detector	RIGAKU RAXIS VII
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	78.389, 47.811, 90.534
Unit cell angles	90.00, 95.54, 90.00

Refinement procedure

Resolution	50.000 - 2.430
R-factor	0.1672
Rwork	0.164
R-free	0.22340
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4xb1
RMSD bond length	0.014
RMSD bond angle	1.748
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	DM

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.520
High resolution limit [Å]	2.430	5.230	2.430
Rmerge	0.045	0.024	0.175
Total number of observations	102119
Number of reflections	25243
<I/σ(I)>	15		4.2
Completeness [%]	99.0	98.4	95.7
Redundancy	4	4	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	PEGME2000, citrate